> Sydowia 77
> Sydowia 76
> Sydowia 75
> Sydowia 74
> Sydowia 73
> Sydowia 72
> Sydowia 71
> Sydowia 70
> Sydowia 69
> Sydowia 68
> Sydowia 67
> Sydowia 66 (2)
> Sydowia 66 (1)
> Sydowia 65 (2)
> Sydowia 65 (1)
> Sydowia 64 (2)
> Sydowia 64 (1)
> Sydowia 63 (2)
> Sydowia 63 (1)
> Sydowia 62 (2)
> Sydowia 62 (1)
> Sydowia 61 (2)
> Sydowia 61 (1)
> Sydowia 60 (2)
> Sydowia 60 (1)
> Sydowia 59 (2)
> Sydowia 59 (1)
> Sydowia 58 (2)
> Sydowia 58 (1)
> Sydowia 57 (2)
> Sydowia 57 (1)

Genomic structure of novel Iranian Rhizoctonia solani AG-3PT isolates on potato, Solanum tuberosum

Dorna Forghani, Eidi Bazgir, Mehdi Nasr Esfahani & Mostafa Darvishnia

Sydowia 73: 217-238

Published online on February 10th, 2021

Rhizoctonia solani AG-3PT is relatively specific to potato, Solanum tuberosum, causing considerable yield losses around the globe, including Iran. A total of 210 R. solani isolates were collected from stem cankers and black scurf of infected potato plant parts in various potato-growing provinces of Iran, of which morphogenetic diversity of 110 R. solani isolates were characterized. High morphological and cultural variation was observed on radial colony growth and colour, hyphae diameter and number of nuclei, and sclerotia initiation, formation and diameter. The fastest mycelium growth rate was in non-pathogenic R. solani isolates. The pathogenicity and virulence characteristics of 70 R. solani isolates varied ranging from 0–100 % based on disease severity. There was no correlation between morphological factors, pathogenicity and geographic origin of the isolates. ITS-rDNA region barcoding of R. solani confirmed the isolates as R. solani AG-3PT. Nineteen polymorphic Inter Simple Sequence Repeat (ISSR) primers were used to reveal the genetic structure and relationships among 110 R. solani isolates, which 258 bands were detected. The average number of bands per primer was 21.5 and the size of the amplified DNA bands ranged from 100–5000 bp, with a high degree of polymorphism (100 %). The majority of the isolates showed nearby associations within the population. UPGMA classified the R. solani isolates into 16 main groups according to geographical origin, indicating that the ISSR marker is a reliable tool in characterizing genetic diversity in R. solani isolates. There was no correlation between ISSR analysis and virulence variability.

Keywords: ISSR, ITS-rDNA, PCR, polymorphism, primer, UPGMA.

eBook at Verlag Berger