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Genetic diversity of Bipolaris spp., the causal agent of rice brown spot: the application of ISSR-based molecular markers

Mohammad Reza Safari Motlagh, Lobna Hajji-Hedfi, Mehdi Khosravi Moghadam, Ali Akbar Ebadi & Ahmed M. Abdel-Azeem

Sydowia 77: 39-52

Published online on August 5th, 2024

The brown spot disease of rice caused by Bipolaris spp. is one of the most important seed-borne diseases of rice in the world. Adequate awareness of pathogens’ genetic structure is highly important for selecting a proper strategy for their control. This research aimed to investigate the genetic diversity of the Bipolaris spp. fungal isolates using the ISSR marker. This study was conducted in Guilan province in Iran for the first time. In this research, a total of 31 fungal isolates of Bipolaris spp. were isolated from rice samples collected from Guilan province and the genetic diversity of B. victoriae was explored by inter simple sequence repeat (ISSR)-based molecular markers. It was found that the species B. victoriae had 97 % frequency and accordingly, 80 bands were obtained out of which 77 gene loci showed polymorphism. ISSR14 with 18 bands and ISSR12 with 14 bands had the highest and lowest number of amplified fragments, respectively. Based on the results, the isolates were classified into four groups in terms of the total number of polymorphic bands of ISSR. The highest genetic diversities were observed in the two groups of leaf and panicle. The results of the genetic diversity of the isolates showed high intra-population diversity. The DNA band patterns could not show a direct correlation between polymorphism and geographical regions. The results revealed high intra-population genetic diversity. Therefore, it is of crucial importance to cultivate resistant cultivars consistent with this diversity in the study location.

Keywords: brown spot disease, ISSR.

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